ZFIN Image: Neumann et al., 2011, Fig. 4

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Figure Caption

Fig. 4

alk6b^W256X is a loss-of-function allele. (A–D) Whole-mount in situ hybridization demonstrating ventral expression of the BMP target gene gata2 in control and morpholino knockdown embryos. Goosecoid (gsc) expression in the prechordal plate marks the presumptive dorsal side of the embryo. (A) Control morpholino injected embryos at 75% epiboly showing normal ventral expression of gata2. (B) Morpholino knockdown of the type IA BMP receptors alk3a and alk3b slightly reduces gata2 expression. (C) Triple knockdown of alk3a, alk3b, and alk6b leads to complete loss gata2 expression. (D) Injection of wild-type alk6b mRNA into triple knockdown embryos rescues gata2 expression. (E) Quantification of percentage of embryos expressing gata2 after morpholino knockdown and rescue with wild-type or alk6b^W256X mRNA.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA