|
Fig. 6 Serial sections of the whole-mount in situ hybridization specimens. A-L: The coronal sections of 24 hours postfertilization (hpf) embryos hybridized to sbno1 (A-C), sbno2a (D-F), delta A (G-I), or HuC (J-L) probes. A,D,G,J: The section 20 to 40 micrometers in depth from the anterior top of the embryos. B,E,H,K: The sections at the anterior midbrain level. C,F,I,L: The sections at the hindbrain level. M,N: The coronal sections of 3 days postfertilization (dpf) larvae at midbrain level hybridized to sbno1 (M) or sbno2a (N) probes. Whole-mount in situ hybridization specimens were embedded in the plastic resin and cut at 10-micrometer thickness. The sections were counterstained by 4′,6-diamidine-2-phenylidole-dihydrochloride (DAPI), and the bright field image showing in situ staining (yellow) and fluorescent image showing DAPI staining (blue) of the same sections were merged. Scale bars = 0.1 mm.