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Fig. 4
Representative images of the effect of diferuloylmethane on TUNEL-labeling in noi mutant larvae at 6 dpf. A: TUNEL-labeling in wholemount larvae (left) and wholemount eye (right) of wildtype zebrafish (wt). B: Extensive TUNEL labeling in larvae and eye of untreated noi mutant zebrafish. C: noi mutant larvae treated with 5 μM diferuloylmethane. D: noi larvae treated with 300 μM zVAD-fmk. Arrow points to optic fissure closure defect delineated by black dotted line. Scale bar whole eye=200 μm; scale bar larval fish=500 μm.
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