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Fig. 3 her8a is regulated by the Su(H)-dependent Notch signaling pathway.
her8a is expressed at lower levels in the Notch activity-deficient mind bomb mutant embryos (C and D) in comparison to wild-type siblings (A and B). B and D are higher power views of hindbrain regions in A and C, respectively. Note the remnants of her8a transcripts in mind bomb mutants at the midbrain and hindbrain rhombomere boundaries (arrowheads in D). (E) Quantification of her8a expression analyzed by qPCR showing significantly reduced level of her8a in mind bomb mutants. (F–H) RNA encoding constitutively-active Su(H) (ANK) (G) or dominant-negative Su(H) (DBM) (H) was injected at the one or two-cell stage and analyzed at the 3-somite stage. (F) Control embryos injected with GFP mRNA; (G) her8a expression is upregulated by constitutive-active Su(H) injection; (H) her8a expression is downregulated in dominant-negative Su(H) injected embryos. (I) The levels of her8a expression in Su(H) variant injected embryos were further quantified by qPCR, showing significantly increased her8a expression in ANK injected embryos and decreased expression in DBM over-expressed embryos. ANK, constitutive-active Su(H); DBM, dominant-negative Su(H), mib: mind bomb mutants.