ZFIN Image: Carvalho et al., 2011, Fig. 1

Image

Figure Caption

Fig. 1

Outcome of M. marinum yolk sac injection of embryos between the 16- and the 512-cell stage.

(A) 5 dpi fli1-egfp larva with gfp-labelled vasculature showing spread of bacteria (red) throughout the body (scale bar: 250 μm). (B and D) Bright-field/fluorescence overlay and (C and E) confocal z-stack of red-fluorescent bacteria showing activation of green-fluorescent gags at the (B and C) edge of the yolk extension and on the (D and E) tail of a 7 day-old larva (scale bar: 25 μm). (F and H) Bright-field confocal plane and (G and I) confocal z-stack of red-fluorescent bacteria co-localizing with green-fluorescent leukocytes detected by L-plastin immunostaining (scale bar: 25 μm). The lesions caused by the granulomas can be clearly seen in F and H.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One