Fig. 4 zfTPP1 interacts with zfTIN2-1 through its C-terminus.
(A) Schematic representation of domain homology between human and zebrafish TIN2. NTD, N-terminal domain. TBM, TRFH-binding motif. (B) zfTPP1 interacts with zfTIN2-1. Extracts from 293T cells co-expressing V5-tagged zfTPP1 or hTPP1, together with FLAG-tagged N terminal part of zfTIN2-1, zfTIN2-2, or zfTIN2-3, were immunoprecipitated with anti-FLAG antibodies and western blotted. FLAG-tagged hTIN2 was also included. (C) The C terminal domain of zfTPP1 mediates its interaction with zfTIN2-1. Extracts from 293T cells co-expressing GST-tagged full-length zfTIN2-1 with FLAG-tagged full-length zfTPP1 or zfTPP1-ΔC were analyzed by immunoprecipitation and western blotting.