ZFIN Image: Xie et al., 2011, Fig. 2

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Figure Caption

Fig. 2 Characterization of zfTPP1.

(A) Schematic representation of domain organization of human and zebrafish TPP1. OB, oligonucleotide/oligosaccharide binding fold. RD, POT1 recruitment domain. S/T, Serine-rich region. TID, TIN2-interacting domain. (B) Expression of TAP-tagged zfTPP1 in zebrafish cells. Whole cell extracts from parental and TAP-tagged zfTPP1 expressing zebrafish cell line ZF4 were western blotted with peroxisome-conjugated protein A. Actin was used as a loading control. (C) Telomere association of zfTPP1. Parental and TAP-tagged zfTPP1 expressing ZF4 cells were crosslinked and used for chromatin immunoprecipitation with protein A beads. The precipitated DNA was analyzed by southern blotting. (D) Quantification of data in D. Error bars indicate standard error (n = 3).

Acknowledgments

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