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Fig. S11 Effect of dorsomorphin on anemia and iron loading in mtp2 deficient embryos. Embryos were injected with mtp2 morpholino at the one cell stage, followed by treatment with dorsomorphin from 28 hpf until fixation for either o-dianisidine staining at 50 hpf (A–D) or whole mount nonheme iron staining at 55 hpf (E–H), lateral views. Uninjected controls (A) and embryos treated with dorsomorphin (B) exhibited normal hemoglobin staining, while mtp2 morphants (C) manifest decreased hemoglobin staining, which failed to improve when mtp2 morphants were treated with dorsomorphin (D). N = 54–99 embryos per group. Compared to uninjected controls (E), embryos treated with dorsomorphin (F), mtp2 morphants (G), or mtp2 morphants treated with dorsomorphin (H) exhibited increased iron staining in the somites, brain, and dorsal spinal cord. N = 32–45 embryos per group. (I,J) Whole mount in situ hybridization for gata1 (lateral views) when embryos have developed 24 somites, about 22 hpf, demonstrated decreased numbers of gata1-staining erythroid precursors in mtp2 morphants compared to uninjected embryos. N = 21–36 embryos per group.