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Fig. S8 Effect of knockdown of RGM genes at 72 hpf. Whole mount in situ hybridization for hepcidin (A–D) or foxa3 (E–H), dorsolateral views. Compared to uninjected controls (A), knockdown of RGMa (B), RGMb (C), or RGMd (D) failed to inhibit hepcidin expression. E–H. Expression of foxa3 in the liver revealed a significant reduction of liver size in the RGMb and RGMd morphants (G, H). N = 20 embryos per group. I. Quantitative real-time RT-PCR revealed no significant decrease in hepcidin transcript levels relative to liver fatty acid binding protein (LFABP). N = 3 pools of embryos per group. Data shown are means + SE.