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Fig. S7 Effect of morpholino knockdown of RGM genes at 55 hpf. Whole mount in situ hybridization for hepcidin (A–D) or foxa3 (E–H), dorsolateral views. Compared to uninjected controls (A), knockdown of RGMa (B), RGMb (C), or RGMd (D) failed to inhibit hepcidin expression (arrow). E–H. Expression of foxa3 in the liver (arrowhead) revealed a slight reduction of liver size in the morphants (F–H) compared to control (E). N = 20 embryos per group.
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