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Fig. S5 Whole mount Alcian blue staining for cartilage in zebrafish embryos at 5 days post-fertilization confirms a branchial arch phenotype in furin morphants. Dorsolateral view of the head of an uninjected control embryo (A) and an embryo injected with morpholinos to knock down furina and furinb (B) reveals an open mouth phenotype (arrow in B) in the furina/furinb morphant. Lateral view of an uninjected control (C) and a furina/furinb morphant showing the fused cartilage elements (arrowhead in D) characteristic of furin morphants. N = 20 embryos per group.
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