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Fig. S1 Treatment with dorsomorphin decreases BMP2b-induced phospho-smad1,5,8 staining in zebrafish embryos. Tg(hsp70:bmp2b) embryos were fixed at 55 hpf for immunohistochemical staining for phospho-smad1,5,8 following (A) no heat shock and no chemical treatment (-HS, -dorso), (B) no heat shock, but treatment with dorsomorphin (-HS, +dorso), (C) heat shock and no chemical treatment (+HS, -dorso), (D) heat shock and treatment with dorsomorphin (+HS, +dorso), representative embryos lateral view. Heat shock was performed at 48 hpf. Dorsomorphin treatment was performed from 28–55 hpf at a concentration of 40 μM. For enhanced sensitivity, a fluorescently-labeled secondary antibody was used (Alexa Fluor® 488 goat anti-rabbit IgG, Invitrogen, #A-11008). Embryos were illuminated with an X-cite Series 120 PC microscope lamp (Exfo Life Sciences and Industrial Division, Quebec, Canada) and emitted light was filtered with a green fluorescent protein (GFP) filter set. N = 15–22 embryos per group.