|
Fig. 4 The hjv transcript is weakly expressed in the zebrafish embryonic liver at the time of hepcidin expression.
A–F. Whole mount in situ hybridization for hjv (A–E) or transferrin-a (F) demonstrating expression in the notochord at 11 hpf (A) (dorsal view), in the somites at 18 hpf (B) (lateral view with yolk removed), but absence from the liver at 50 hpf (C), 72 hpf (D), and 7 days post-fertilization (dpf) (E). In comparison, transferrin-a is strongly expressed in the liver at 7 dpf (F). N = 10–30 embryos per group. G. Semiquantitative RT-PCR for hepcidin, hjv, and transferrin-a expression in embryonic zebrafish hepatocytes (top, GFP+) and nonhepatocytes (top, GFP-) and for hepcidin and hjv in adult zebrafish skeletal muscle and liver (bottom). Embryonic hepatocytes were sorted by FACS from pools of 80–100 transgenic zebrafish embryos at 72 hpf, which express GFP under the control of the liver-specific LFABP promoter. RT- indicates control reaction with reverse transcriptase omitted.