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Fig. 4 her9 inhibits Cdkns and controls cell proliferation. (A-D) In situ hybridization of cdkn1bl (A,B) and cdkn1c (C,D) in wild-type (A,C) and her9-MO-injected (B,D) zebrafish embryos at 24 hpf. Arrow indicates posterolateral expansion of cdkn1bl expression. (E-H) Transverse sections of wild-type and Her9 morphant embryos stained by in situ hybridization for her9 (blue) and immunohistochemistry with anti-pH3 (green). (I) Proportion of pH3-positive cells counted inside and outside the her9-expression domain in wild-type and Her9-depleted embryos. Error bars indicate mean + s.e.m. *P=0.001. A-D and E-H are at the same magnification.