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Fig. 5 Detection of Cre-mediated site-specific deletion by PCR analysis. A: Scheme of the expected PCR products before (1,523 bp) and after (304 bp) Cre-mediated recombination in the red-to-green reporter line using the primers indicated as arrows. B: Cre-mediated recombination of the (hsp70l:mCherry-T2A-CreERT2)#15 allele in the red-to-green reporter line in the absence (-) or presence (+) of heat and/or TAM at different developmental stages (mid-gastrulation, 1 day, 5 days, 10 days). In the absence of heat and TAM, only the unrecombined product of 1,523 bp can be detected, whereas application of heat or TAM or both leads to the amplification of the recombined 304-bp fragment. kb, kilobase.