ZFIN Image: Hans et al., 2011, Fig. 2

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Figure Caption

Fig. 2 RT-PCR reaction analysis of the Tg(hsp70l:Cre), the Tg(hsp70l:EGFP-Cre), the Tg(hsp70l:fgf8a), and the Tg(hsp70l:dnfgfr1-EGFP) alleles as well as the endogenous hsp70l gene at permissive temperatures. A: When the Tg(hsp70l:Cre) allele is provided paternally, no Cre transcripts can be detected at the 2-cell stage but at late blastula stages and 24 hpf. In contrast, maternal contribution of the Tg(hsp70l:Cre) allele results in the detection of Cre transcripts at all examined stages. B: Similarly, if the Tg(hsp70l:EGFP-Cre) allele is provided paternally, Cre transcript can not be detected at the 2-cell stage but at late blastula stages and 24 hpf, whereas maternal contribution of the Tg(hsp70l:Cre) allele is present at all stages. C: Maternal contribution of the Tg(hsp70l:fgf8a) and the Tg(hsp70l:dnfgfr1-EGFP) alleles result in the detection of fgf8a and EGFP transcripts at the 2-cell stage. In wild-type embryos, hsp70l can be detected at the 2-cell stage and at 24 hpf.

Acknowledgments

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