ZFIN Image: Qin et al., 2009, Fig. S1

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Figure Caption

Fig. S1 Isolation of GFP⁺ Müller glia. (A) Dissociated GFP⁺ Müller glial cell (green). Counterstained with DAPI (blue). (B and C) Flow cytometry scatter plots. forward scatter-height (FSC-H); side scatter-height (SSC-H). Dissociated cells from adult Tg(gfap:GFP)mi2002 zebrafish retinas were gated by forward and side scatters (B), and GFP⁺ Müller glia were isolated based on fluorescence in the FITC channel (R5) (C). Our yield of dissociated retinal cells from adult zebrafish (5- to 6-month old) was ~2.5 x 10⁵ cells/retina, of which ~9% were GFP⁺ Müller glia. With flow cytometry, we could recover ~2.1 x 10⁴ Müller glia/retina, representing an efficiency of ~84%. (Scale bar: 10 μm.)

Acknowledgments

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