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Fig. 2 A katna1 splice-blocking (katna1e4i4) morpholino inhibits spinal motor axon outgrowth in a p53-independent manner. Embryos were injected with 1.0 pmol CoMO + 1.6 pmol p53MO (A), 0.8 pmol katna1e4i4 + 1.6 pmol p53MO (B) or 1.0 pmol spg4e7i7 + 1.6 pmol p53MO (C), fixed at 28 hpf, and then immunostained with znp-1. Both katna1 and spast splice-blocking morpholinos inhibit spinal motor axon outgrowth in a p53-independent manner (D). CoMO + p53MO (n=25), spg4e7i7 + p53MO (n=35), katna1e4i4 (n=34); statistical significance was determined using ANOVA with Bonferroni’s multiple comparison test. ***P<0.001. Scale bars: 50 μm.