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Fig. 4 Sox17 and chd are required for left-right asymmetry determination in zebrafish. Embryos were injected into the yolk at one-cell (C) or midblastula (A,B,D,E) stages, with CO MO (B,C,E), sox17 MO (A and B,C), or chd MO (D and E) as indicated in the bar graphs. Whole mount in situ hybridization for spaw (A,D: arrowheads, 15-18 somite; dorsal view with anterior on top) showed aberrant expression when either sox17 MO or chd MO was injected, as summarized in B,C, and E. L, left; B, bilateral; R, right; A, absent. Number of embryos is shown at the top.
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