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Fig. 2 Disruption of acvrl1 increases the number of endothelial cells in the basal communicating artery (BCA) and posterior connecting segments (PCS) at 2-2.25 dpf. (A,B) Representative confocal micrograph of the central cranial vasculature of (A) a vbg+;TG(fli1;nEGFP)y7 wild-type embryo, and (B) a vbgft09e;TG(fli1;nEGFP)y7 mutant embryo. The greater than two-fold increase in endothelial cell number in the BCA/PCS in vbgft09e embryos compared to wild-type embryos is significant at P<0.00001. BA, basilar artery. Dorsal views, anterior to the left.
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