ZFIN Image: Pittman et al., 2010, Fig. 8

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Figure Caption

Fig. 8 cyfip2 acts cell autonomously in dorsonasal RGC axon sorting in the optic tract. (A) Co-electroporation yields consistent co-labeling with EGFP and mCherry. (B) Diagram of rescue experiments: electroporated dorsonasal RGCs project to the posteroventral quadrant of the tectum. (C) Quantification of D-V sorting of dorsonasal axons in the optic tract as they enter the right optic tectum, relative to the ventralmost (0%) and dorsalmost (100%) fascicles (white ruler in D). A subset of nev axons missorts into the dorsal brachium; electroporation with cyfip2 yields significant rescue. Error bars show mean ± SEM. (D–F) Confocal projections of single dorsonasal axons (GAP43-GFP, green) in the tectal neuropil (mCherryCAAX, red) at 5 dpf. (D) WT axons enter the tectum ventrally. (E) nev axons often enter dorsally. (F) cyfip2-co-electroporated nev axons are rescued and enter ventrally. Scale bar, 50 μm.

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Acknowledgments

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Reprinted from Developmental Biology, 344(2), Pittman, A.J., Gaynes, J.A., and Chien, C.B., nev (cyfip2) Is required for retinal lamination and axon guidance in the zebrafish retinotectal system, 784-794, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.