Fig. S2

Fig. S2 Control experiments of Prep1.2 morpholinos. A: An embryo injected with a 6 bases mismatched prep1.2 morpholino develops two posterior streams of NCC (arrowheads) as evidenced by dlx2a expression pattern. B, C: loss of dlx2a expression in the posterior stream of NCC in embryos injected with MO2-prep1.2 is rescued when a prep1.2 mRNA lacking the morpholino target sequence is co-injected. D: An embryo injected with a 6 bases mismatched prep1.2 morpholino develops normal splanchnocranium. E, F: Rescue of defects in the posterior branchial arches of MO2-prep1.2 morphants by coinjection of a prep1.2 mRNA lacking the morpholino target sequence. G–I: Acridine orange staining in living embryos injected with prep1.2 morpholinos does reveal few p53-independent (G, n = 23) or p53-dependent (H, n = 27) cell death.