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Fig. 2 Localization of GPER in zebrafish ovarian tissue cryosection samples by immunohistochemistry using a specific GPER antibody. (A–D) Early-vitellogenic stage follicles. (E–H) Late-vitellogenic stage follicles. (A, E) Green fluorescent staining of zebrafish GPER on oocyte plasma membranes (om). (B, F) DAPI staining of nuclear DNA of follicular cells (f). (C, G) Merge. (D, H) Detection of GPER with the GPER antibody that had been neutralized with the antigen peptide and merged with DAPI stained images. Scale bar = 100 μm.
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Reprinted from Developmental Biology, 342(2), Pang, Y., and Thomas, P., Role of G protein-coupled estrogen receptor 1, GPER, in inhibition of oocyte maturation by endogenous estrogens in zebrafish, 194-206, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.