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Fig. 1 Expression profile of ovo1. Wholemount in situ hybridization. (A) Enveloping layer (EVL) expression at 5 hours post-fertilization (hpf). (B) Neural crest (NC) and prechordal plate (PCP) expression at 10 hpf (dorsal view, anterior to the left). (C,D) Expression in premigratory cranial NC cells at 12 hpf (C, dorsal view; D, lateral view). (E,F) Expression in migrating cranial NC cells at 15 and 24 hpf (dorsal views). Additional expression at 24 hpf in otic vesicles (ot) and roof plate (rp; F, arrows). (G,H) Expression at 48 hpf in pharyngeal arches (pa), pectoral fins (pf), otic vesicles and neurocranium (ne; G, lateral view; H, ventral view). e, eye.