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Fig. 1 miR-143 is expressed in the zebrafish embryonic heart. Whole-mount in situ hybridization for miR-143. (A,B) At 36 and 38 hpf, miR-143 is expressed throughout the heart tube and outflow tract (OFT). Anterior views. (C,D) At 48 hpf, miR-143 is expressed in the ventricle and OFT. Lateral view (C) and magnification of the heart (D). (E) Ventricular miR-143 expression at 72 hpf; OFT expression is high at this stage (arrowhead). Expression is also observed in pharyngeal cells. (F) At 5 dpf, transcripts are detected in the ventricle, OFT and in a population of pharyngeal cells. Plastic sections. (G) Magnification of the boxed region in F; miR-143 transcripts are detected throughout the ventricular wall. O, outflow tract; V, ventricle; A, atrium; Lu, lumen.