ZFIN Image: Christie et al., 2010, Fig. 4

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Fig. 4 syk rescues angioblast migration defects after syk knockdown. (A–D) syk and zap-70 are expressed in a ubiquitous manner and have overlapping expression profiles. At 16 somites (16s) syk (A) and zap-70 (C) are highly expressed in the head, with low levels of expression throughout the rest of the embryo. (B, D) By 21s increased expression is seen in the ventral trunk. (E–F) By 30 hpf, the ISVs of wildtype Tg(fli1:egfp)^y1 embryos have reached the dorsal portion of the embryo to form the DLAV (E) while the ISVs of syk morphants are delayed at the mid-trunk (F). (G–H) Expression of a C-terminal myc-tagged syk in the vasculature (G, fli1:syk:myc) or blood (H, gata1:syk:myc) partially rescues the number of ISVs that have reached the DLAV (black arrow).

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Acknowledgments

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Reprinted from Developmental Biology, 340(1), Christie, T.L., Carter, A., Rollins, E.L., and Childs, S.J., Syk and Zap-70 function redundantly to promote angioblast migration, 22-29, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.