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Fig. 3
Responsiveness of Tg(lhx1a:EGFP)pt303 to perturbations in Nodal signaling.
(A-H) Shield stage Tg(lhx1a:EGFP)pt303 embryos. (A-C,E-G) Animal view. (D,H) Lateral view. (A,E) Uninjected control embryos. (B,F) Embryos injected with 125pg mCherry RNA. (C,D,G,H) Embryos injected with 100pg mCherry RNA and 25pg ndr1 RNA. (A-D) Tg(lhx1a:EGFP)pt303 expression. (B) Overlay of mCherry expression (red) displays an embryo that was successfully injected. (E-H) In situ hybridization for lhx1a.
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