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Fig. 3
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Fig. 3 RT-PCR analyses of NITR gene expression from different leukocyte populations. Lymphoid and myeloid cell populations were purified from the kidney of multiple zebrafish and pooled as described (Yoder et al. 2007). RT-PCR was performed as described in Fig. 2, except 1 μg of total RNA was used in the reverse transcription reaction. RT-PCR of myeloperoxidase (mpx) provides a positive control for myeloid cells and TCR-α provides a positive control for T lymphocytes. β-actin is shown as a standard reference. Primer sequences and thermal cycling parameters are listed in Table 1
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