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Fig. 5 Irxl1 morphants show abnormal brain morphogenesis. Wild-type (A,D,G,J) and irxl1 MOI (B,E,H,K), and MOII (C,F,I,L) morphant embryos at 24 hours postfertilization (hpf). The p53 MO was co-injected with MOI. A-C,G-L: Lateral views. D-F: Dorsal views. A-F: Brain morphology of live embryos. The heads of ixl1 morphants become small and flat, and the ventricles fail to inflate properly (arrows in E,F). G-L: Whole-mount in situ hybridization with wnt1 (G-I) and krox20 (J-L) reveals defective segmentation of the hindbrain (arrows in H,I) and reduced expansion of the brain in the anterior-posterior and dorsal-ventral directions (double arrows in K, L) in morphants. di, diencephalon; e, eye; h, hindbrain; m, midbrain; mhb, mid-hindbrain boundary; r, rhombomere; te, telencephalon. Scale bar = 100 μm.