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Fig. 3 col27a1b is expressed in notochord and cartilage.
col27a1b was expressed throughout the notochord at 13 somites (A) but by 24 hpf (B) was restricted to the distal tip of the tail (black arrow). (E) col27a1b expression in the notochord was restricted to the notochord itself and was not present in surrounding structures. (F) Expression of col27a1b in cartilage was observed beginning at 48hpf. Cartilage expression was also seen at 3 dpf (C, G) and 5 dpf (D, H), but it was diffuse and individual cartilage structures were not easily distinguished. Scale bar in E represents 20 μm. Scale bars in remaining images represent 100 μm. Scale bar in C applies to D, G, and H. cb, ceratobranchial arches; ch, ceratohyal; cl, cleithrum; d, dentary; e, ear; ep, ethmoid plate; m, maxilla.