Fig. S1 Phenotypes of arl13b morphants. (A) Injected at modest dosage, arl13 morphants show similar phenotypes to scohi459 mutants. The morphants can be rescued by co-injection of arl13b mRNA. c-mo, embryos injected with a standard control morpholino; sco-mo, embryos injected with the arl13b morpholino; sco-mo+mRNA, embryos co-injected with arl13b morpholino and mRNA. (B) Injected at higher dosage, arl13 morphants show more severe phenotypes at 1 dpf. mph, morphant; wt, wild type. (C) A shield-stage embryo injected with FM1-43 in dorsal view. (D,E) Side views of injected embryos at the tailbud stage. Arrows point to the tip of the head and the tail. mo+sco, embryos co-injected with sco morpholino and mRNA (D); mo+gfp, embryos co-injected with sco morpholino and eGFP mRNA (E).
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