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Fig. S8 Apical-basal cell polarity after FgfR inhibition. (A and B) Normal localization of the apical membrane markers aPKC (A) and ZO1 (B) at 10ss, after FgfR-inh. treatment (bottom panels) compared to control embryos (top panels). (C and D) Cell delamination in the optic vesicle after FgfR-inh. treatment (D) compared to control (C) at 15ss. Sites of delamination (arrowheads in [D]) correspond to regions where apical membrane contact between dorsal and ventral leaflet (revealed by staining for aPKC, green) is lost. Images are transverse sections, counterstained with DAPI (blue) and for F-actin (red), dorsal to the top, dotted lines: neural tube boundary. d, dorsal optic vesicle leaflet; v, ventral optic vesicle leaflet.