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Fig. 6 Knockdown of scinlb expression by injection of a specific morpholino oligonucleotide (MO). A,B: The scinlb-ATG MO effectiveness assay. C,D: Abnormal development of brain in scinlb-ATG MO microinjected embryos at 29 hours postfertilization (hpf). E,F: Increased cell death in brain and neural tube of scinlb morphant detected by Acridine Orange staining. Arrows represent similar regions. G,H: Decreased expression of shhb in the scinlb morphant. Three separate experiments were conducted, and ∼100 embryos were analyzed each time.
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