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Fig. 7 Dmrt2b knockdown disrupts L–R identity in lateral plate mesoderm.
(A–D) lefty1 normal expression in left dorsal diencephalon at 22 somites stage were disrupted embryos injected with Dmrt2b-MO. (E–H) lefty1 normal expression in left LPM at 22 somites stage were disrupted embryos injected with Dmrt2b-MO. (Q) Graphical representation of the percentage of embryos exhibiting the expression patterns for lefty1 in Dmrt2b knockdown embryos and control embryos. (I–L) spaw normal expression in left LPM at 20 somites stage were disrupted embryos injected with Dmrt2b-MO. (R) Graphical representation of the percentage of embryos exhibiting the expression patterns for spaw in Dmrt2b knockdown embryos and control embryos. (M–P) pitx2c normal expression in left LPM at 22 somites stage were disrupted embryos injected with Dmrt2b-MO. (S) Graphical representation of the percentage of embryos exhibiting the expression patterns for pitx2c in Dmrt2b knockdown embryos and control embryos. (T) Lateral view of the embryos at 22 somites stage. Expression of the ntl in embryos injected with Dmrt2b-MO is similar to expression of ntl in control embryos. Results are represented as mean±SD of three separate experiments. (U) qPCR analysis of the expression changes of ntl in 24 hpf embryos injected with Cont-MO or Dmrt2b-MO. The data represents mean±SD of three separate experiments.