|
Fig. S3 BODIPY-ceramide-labeled vesicular structures in control and Cdh11 knockdown 42-hpf embryos. BODIPY-ceramide-labeled living 42-hpf zebrafish embryos injected with control morpholino oligonucleotide (A) and with cdh11 antisense morpholino oligonucleotide (B). Confocal optical sections are shown, using a Zeiss LSM 510, C-apochromat 63x W 1.2 NA objective, mounted in embryo medium. Representative confocal image planes are shown. Due to technical difficulties described in the text, quantification would not be as reliable at this developmental stage. However, there was an apparent increase in the number and size of BODIPY-labeled puncta in Cdh11 knockdown embryos. Scale bar = 30 μm.