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Figure Caption
Fig. S4 ints5 morpholinos lead to truncated Smad5 protein in embryos. Western blots on extracts of embryos injected with control or ints5 morpholinos. Proteins were harvested from 20 injected embryos at 8 hpf. Smad5 proteins were detected using rabbit anti-Smad5 polyclonal antibody (upper panel); the lower panel shows expression of α-Tubulin control detected with mouse monoclonal anti-α-Tubulin antibodies. The black arrows indicate the size of corresponding peptides.
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