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Fig. S2 Knockdown of ints5 does not affect germ-layer specification. (A-H) Whole-mount in situ hybridization to detect expression of notail (ntl) (A-D) and spadetail (spt) (E,F) at 50% epiboly (5.3 hpf) and 75% epiboly (8 hpf), respectively, and goosecoid (gsc) (G,H) in shield-stage embryos (6 hpf), injected with ints5 morpholinos (C,D,F,H), or control morpholinos (A,B,E,G). Open arrowheads in E and F indicate the distance between spt-positive cells in the midline. (I-L) Expression of dlx3 (I,J) at the neural plate boundary in tailbud stage (TB, 10 hpf) embryos, and sox17 (K,L) in the endoderm of 75% epiboly embryos injected with ints5 morpholinos (J,L) or control morpholinos (I,K). Black arrows in I and J mark the distance between the boundary of dlx3 expression. A and C show lateral views of embryos with animal pole to the top; B and D show animal pole views; E-L show dorsal views of embryos with animal pole to the top. The total number of embryos for each marker was >50, from three independent injection experiments. Scale bar: 50 μm.