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Figure Caption
Fig. S1 ints5 morpholinos abolish Ints5 protein in embryos. Western blots on extracts of embryos injected with lacZ RNA, ints5 RNA or ints5 morpholinos. Proteins of each sample were harvested from 20 injected embryos at 8 hpf. Ints5 proteins were detected by using rabbit anti-Ints5 antibody (upper panel); lower panel shows expression of α-Tubulin control detected using anti-α-Tubulin antibodies.
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