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Fig. 7 Knockdown of ints11 perturbs smad5 splicing. (A)RT-PCR analysis shows that the ints11 splice-junction morpholino efficiently blocks the correct splicing of ints11 RNA, and several aberrant transcripts are detected. Transcripts are indicated by black arrows, with sizes indicated. (B) Schematic representation of the ints11 genomic locus (upper panel); schematic representation of normal or aberrant ints11 transcripts is shown in the lower panel. Numbered black arrows indicate the position of primers used in RT-PCRs to detect splicing. Orange bar shows the target site of the ints11 splice-site morpholino. (C)RT-PCR analysis shows that splicing of smad5 is impaired in ints11 morphants. (D) Schematic representation of the smad5 genomic locus (upper panel); the lower panel shows schematic representations of normal and aberrantly spliced smad5 transcripts.