Fig. S1 Convergent extension defects at bud stage and 48 hours. Injected embryos were scored at bud stage and again at 48 hours to determine the extent of the CE defect. At bud stage, the angle between the tailbud and head was measured. An angle greater than 90 degrees was scored as WT and less than 90 degrees as a CE phenotype. At 48 hours, short embryos with misshapen somites were scored as a CE phenotype. The angle between the head and tailbud in (A) a WT and (B) a morphant embryo. (C) Phenotype of WT (112 embryos), morphant (109 embryos), rescued embryos (121 embryos), cpi-17 (95 embryos) and ca-mlck (121 embryos) injected embryos were scored twice, once at bud stage and again at 48 hours.
Image
Figure Caption
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Development