Fig. 5

Fig. 5 TTLL3 Is Required for Tubulin Glycylation and Cilia Assembly in Zebrafish

(A–F) Images of live control and morphant embryos at 24 (A and B), 48 (E and F), and 55 hpf (C and D). Hydrocephaly is apparent in TTLL3-ATG morphant embryos, as indicated by the enlarged vesicles in the hindbrain (B and F, arrows).

(G) The head of a WT embryo stained by 6-11 B1 antibodies at 72 hpf (the olfactory placode is marked with an arrow).

(H–I″) In olfactory cilia, TTLL3-ATG MOs reduce the levels of tubulin monoglycylation detected by TAP952 mAb at 72 hpf (compare [H] and [I]), but not the levels of tubulin polyglutamylation revealed by polyE antibodies (H′ and I″).

(J and K) The olfactory cilia stained with AXO49 anti-polyglycylated tubulin mAb in a control (J) and a TTLL3-ATG morphant (K) at 72 hpf.

(L and M) Olfactory cilia stained with 6-11 B-1 mAb in a control (L) and a TTLL3-ATG morphant (M) at 72 hpf.

(N–R) SEM images of the zebrafish olfactory placode at 72 hpf in control (N and O) and a TTLL3-ATG morphant (P and R); (O) and (R) are higher magnifications of areas of placodes shown in (N) and (P).

(S) TEM image of a fragment of the olfactory placode in the TTLL3-ATG embryo.