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Fig. S1 Binding assays and expression of Pak1 constructs (A) Co-immunoprecipitation of whole cell lysates overexpressing HA-LC8 and Myc-Pak1, and associated mutants. Results indicate mutation of the LC8 binding site in Pak1 abrogates the interaction. (B) Western blot of GFP-Pak1 constructs in MCF-7 cells.
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