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Fig. 3 Expression of endothelial cell-specific adhesion molecule (ESAM) as analyzed by in situ hybridization. A,D: Dorsal view, anterior is to the left. B,C,E: Lateral view, anterior is to the left. A-C: A flat-mounted 15-somite embryo (A), whole-mounted 22-somite (B), and 24 hours postfertilization (hpf; C) embryos; higher magnification view of the tail region (inset in C). D-G: Anterior region of a flat-mounted 24 hpf embryo (D), 48 hpf stage embryos (E-G), views of esam expression in epiphysis (F) and heart (G). A,B: Note esam expression in the progenitors of head vessels (hv), endocardium (ec), dorsal aorta (da), and posterior cardinal vein (pcv), as well as nonvascular expression in the otic vesicle (ov). C-E,G: Esam is expressed in the axial, intersegmental (isv), and head vessels (C); and in the dorsal longitudinal vessel, aortic arches, and endocardial cells of the atrium (a) and the ventricle (v; D,E,G). C,E,F: In addition, esam is expressed in the otic vesicle (arrows, C,E) and a subset of neurons in the brain region including epiphysis (ep; C,E,F).