ZFIN Image: Bae et al., 2009, Fig. 13

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Fig. 13 Cerebellar mutants. (A–E) Wild-type, (F–J) evanescence (eva), (K–O) asynergy (asy), (P–T) gazami (gaz), and (U–Y) shiomaneki (sio) mutant larvae at 5 dpf. The embryos and larvae were treated with phenylthiourea to prevent pigmentation. The fixed 5-dpf larvae were stained with anti-Pvalb7 and Vglut1 antibodies. Bright-field images of the entire body (A, F, K, P, U) and the head region (B, G, L, Q, V). Lateral views with anterior to the left. Pvalb7 immunostaining (green; C, H, M, R, W), Vglut1 immunostaining (magenta; D, I, N, S, X), and merged images (E, J, O, T, Y). Dorsal views with anterior to the left. The eva and asy mutants had small eyes (indicated by arrows). Scale bars: 1 mm (U) and 200 μm (V, Y).

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Acknowledgments

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Reprinted from Developmental Biology, 330(2), Bae, Y.K., Kani, S., Shimizu, T., Tanabe, K., Nojima, H., Kimura, Y., Higashijima, S.I., and Hibi, M., Anatomy of zebrafish cerebellum and screen for mutations affecting its development, 406-426, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.