Fig. 5

Fig. 5 olig2⁺ cells. (A) Expression of olig2 in the adult cerebellum. A sagittal section. (B, C) Co-staining of a Tg(olig2:EGFP) brain with anti-Pvalb7 (magenta) and anti-EGFP (green) antibodies. Low (B) and high (C) magnification views. Note that the EGFP signals never overlap with Pvalb7. The ventricular zone (VZ) is indicated by a dotted line (B). (D–F) Co-staining of the Tg(olig2:EGFP) brain with anti-EGFP antibodies (green), and anti-Sox10 (D), anti-HuC/D (E), or anti-Mbp (F, magenta). Sagittal sections and in the posterior CCe (D), and anterior CCe (E) and GCL (F). Note that olig2:EGFP⁺ cells that have a small size were stained with anti-Sox10 antibody (arrowheads in D); those having a large cell size were stained with anti-HuC/D antibody (arrowheads in E). olig2:EGFP⁺ cellular processes do not overlap with Mbp⁺ oligodendrocytic processes, but rather are adjacent to them (arrowheads in F). (G) BrdU incorporation. Adult Tg(olig2:EGFP) fish were labeled with bromodeoxyuridine (BrdU) for 1 h, and the proliferating cells were analyzed by immunostaining with anti-BrdU (magenta) and anti-EGFP antibodies (green). A sagittal section. The VZ is indicated a dotted line. Note that some of olig2:EGFP⁺ cells incorporated BrdU (arrowheads). (H) Co-staining of the Tg(olig2:EGFP) brain with a gad1/gad2 riboprobe (magenta) and anti-EGFP antibody (green). olig2:EGFP signals do not overlap with gad1/2 expression. (I) Co-staining of the Tg(olig2:EGFP) cerebellum with a vglut2a riboprobe (purple, NBT/BCIP staining) and anti-EGFP antibodies (brown, DAB staining). Note that some of the olig2:EGFP⁺ cells express vglut2a. (J) Co-staining of the Tg(olig2:EGFP) cerebellum with anti-Pvalb7 (magenta) and anti-EGFP (green) antibodies. Note that some of olig2:EGFP⁺ somata (arrows) receive synaptic inputs from Pvalb7⁺ axons of Purkinje cells. The olig2:EGFP^- eurydendroid cells are marked by asterisks. (K, L) Retrograde labeling of eurydendroid cells. The neural tracer was injected into the pretectal region (asterisk, K) of the Tg(olig2:EGFP) adult fish and the brain was stained with the fluorescent avidin (magenta) and anti-EGFP antibodies. (L) High-magnification view of the box in K. Note that an olig2:EGFP⁺ cell (arrowhead) incorporated the neural tracer. The abbreviations are described in Fig. 1. Scale bars: 200 μm (A, B, K), 50 μm (D, E, G, I), and 20 μm (C, F, H, J, L).