Fig. 3

Fig. 3 Zash-1a expression in wild-type embryos. 12-hr (A), 14-hr (B), 20-hr (C), 24-hr (D), 30-hr (E, F), and 26-hr (G) embryos were hybridized with a Zash-1a antisense probe. (A-E) Lateral views of whole-mounted embryos. (F) Dorsal view of a 30-hr whole-mounted embryo. (G) Lateral view of a thick longitudinal section of a 26-hr embryo (eyes and lateral head removed) observed by DIC optics (montage of three photographs taken at slightly different planes of focus). Symbols indicating expression sites: open triangles, ventral forebrain; asterisks, epiphysis; open stars, hindbrain-midbrain boundary; small arrowheads, rows of expressing cells in the dorsal hindbrain; open arrows, ventral cells near floor plate of cerebellum and rhombomere1; thin arrows, tegmentum; large arrowheads, cells outside CNS flanking anterior hindbrain; thick short arrows, neural retina; medium arrowheads, expressing cells in spinal cord; filled stars, diffuse staining near yolk and gut. Abbreviations: c, cerebellum; d, diencephalons; ep, epiphysis; h, hypothalamus; or, optic recess; o, otocyst; r1...r6, rhombomeres 1 through 6; t, telencephalon, tg, tegmentum; tt, tectum; tv, tectal vesicle, y, yolk. Scale bars in A and G, 100 μm (A-F at same magnification).