ZFIN Image: Chen et al., 2009, Fig. 7

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Figure Caption

Fig. 7 Overexpression of cdx1b increased goblet cell numbers in the intestine. A-D: Wild type (A), lacZ mRNA-injected (B), and cdx1b mRNA-injected (C, D) 98-hr post-fertilization (hpf) deyolked embryos hybridized with agr2 antisense RNA probes. E-H: Wild type (E), CMV-GFP-injected (F), and CMV-cdx1b-injected (G, H) 98-hpf deyolked embryos stained with alcian blue. The arrowhead in G indicates secreted mucin in the lumen. I: Comparison of agr2-expressing goblet cell numbers in the intestine of lacZ (n = 20) and cdx1b (n = 20) ectopically expressed embryos. J: Comparison of alcian blue-stained goblet cell numbers in the intestine of GFP (n = 20) and cdx1b (n = 20) ectopically expressed embryos. Error bars indicate standard errors. *P < 0.001. Gc, goblet cell. Scale bars =100 μm.

Acknowledgments

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