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Fig. 7

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ZDB-IMAGE-090504-43
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Figures for Glasgow et al., 1997
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Fig. 7 Expression of Lim3 in the epiphysis is restricted to a subset of projection neurons. (A–C) Four to five Lim3-expressing cells are seen on each side of the midline in a ventrolateral position in the epiphysis of a 28-h embryo. (A) Dorsal view. (B) Lateral view. (C) Anterior view. This optical section through the epiphysis, with the diencephalon in focus, is slightly deeper than in Fig. 5D. (D) Dorsal view of a 2-day-old embryo. Whole-mount embryos were double labeled with anti-S-antigen (red) and anti-Lim3 (blue, nuclei). Four to five projection neurons express Lim3 protein. Several cells, especially in the center of the epiphysis, express the photoreceptor marker, S-antigen (arrow). By this stage several prominent melanocytes are seen. Scale bar is 50 μm.

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Reprinted from Developmental Biology, 192, Glasgow, E., Karavanov, A.A., and Dawid, I.B., Neuronal and neuroendocrine expression of lim3, a LIM class homeobox gene, is altered in mutant zebrafish with axial signaling defects, 405-419, Copyright (1997) with permission from Elsevier. Full text @ Dev. Biol.