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Fig. 2 cxcr4b is a marker of habenular progenitors/early-born neurons. (A-B″) Single confocal images (dorsal views) of a 48 hpf Tg(huC:gfp) zebrafish embryo (A-A″) with in situ labelling of ngn1 and a 48 hpf Tg(ngn1:gfp) embryo (B-B″) with in situ labelling of cxcr4b. (A″-B″) Merged images. The habenulae are indicated by a dashed white line. (C-E) Cross-sections of double-labelled habenulae generated from confocal z-series. (C) Immuno-detection of GFP protein (green) and localisation of cxcr4b transcripts (red) in a 48 hpf Tg(huC:gfp) embryo. (D) Immuno-detection of GFP protein (green) and localisation of ngn1 mRNA (red) in a 48 hpf Tg(huC:gfp) transgenic embryo. (E) Immuno-detection of BrdU (red) and GFP protein (green) in a 60 hpf Tg(huC:gfp) embryo pulsed with BrdU at 48 hpf.