|
Fig. S4 Efficiency of Sim1 and Olig2 temperature- inducible constructs. Embryos were injected with either HSE:Sim1/GFP (B,E) or HSE:Olig2/GFP (C,F) DNA constructs. At 5 hours post fertilization (hpf), embryos were incubated at either a non-permissive temperature (28° C; A,D) or a permissive temperature (37°C; B,C,E,F) for 45 minutes, to induce gene expression. Embryos at 7 hpf stage were analyzed for expression of sim1 (B) or olig2 (C) by whole mount in-situ hybridization using sim1 and olig2 RNA probes. (D-F) Projected confocal z-stack images (24hpf) of GFP expression driven by the bi-directional heat shock element (HSE). Note that the contrast was artificially enhanced in image displaying the control embryo (panel D) to compensate for complete lack of fluorescence. Dien, diencephalon; e, eye. Scale bars: (A-C) 100 μm; (D-F) 22 μm.